Stanford Genome Evolution Center

Stickleback BAC library:

 

A large-insert stickleback genomic bacterial artificial chromosome (BAC) library has been prepared from a mixed population of anadromous fish from the Salmon River in British Columbia. Large insert DNA was prepared from blood cells, partially digested with EcoR1, ligated to the pTARBAC2.1 vector, and transformed into bacteria. A total of 72,000 clones were plated, picked, and arrayed in 384 well plates. Based on the average size of the inserts and the estimated size of the stickleback genome, the 72,000 clones should represent approximately a 15 to 20x genomic library for Gasterosteus aculeatus. Many thanks to Dolph Schluter for providing fish, Chris Amemiya for preparing large insert DNA fragments from blood cells, and Pieter DeJong's group at the Children's Hospital Oakland Research Institute (CHORI) for building and arraying the library.

 

We are making the stickleback library publicly available through the storage and distribution mechanism of BACPAC resources at CHORI. For more information on filter sets, individual clones, and copies of the libraries please see:

 

http://bacpac.chori.org/threesp213.htm

Note that the entire library can be screened by ordering sets of four filters from library CHORI-213 (18,000 clones per filter). We have had excellent success with the overgo hybridization method described in the "Information and Protocols" section of the CHORI BAC/PAC Resources web site.